This paper presents a new method for instantaneous pitch frequency determination from speech signals. The proposed method is based on the variational mode decomposition (VMD) and the Hilbert transform. The VMD is applied in iterative way with specific input parameters in order to determine the fundamental frequency component from the speech signals. The fundamental frequency component has been used for detection of voiced and non-voiced regions from speech signals. The instantaneous pitch frequency is computed using Hilbert transform of the fundamental frequency component corresponding to voiced regions of speech signals. The experimental results are shown on speech signals taken from Keele pitch extraction reference database. The experimental results obtained from the proposed method are compared with the other existing methods for determining pitch frequency from speech signals.