A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments
J Messing, J Vieira - Gene, 1982 - Elsevier
The strategy of shotgun cloning with M13 is based on obtaining random fragments used for
the rapid accumulation of sequence data. A strategy, however, is sometimes needed for
obtaining subcloned sequences preferentially out of a mixture of fragments. Shotgun
sequencing experiments have shown that not all DNA fragments are obtained with the same
frequency and that the redundant information increases during the last third of a sequencing
project. In addition, experiments have shown that particular fragments are obtained more …
the rapid accumulation of sequence data. A strategy, however, is sometimes needed for
obtaining subcloned sequences preferentially out of a mixture of fragments. Shotgun
sequencing experiments have shown that not all DNA fragments are obtained with the same
frequency and that the redundant information increases during the last third of a sequencing
project. In addition, experiments have shown that particular fragments are obtained more …
[引用][C] A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments. 1982
J Messing, J Vieira - Biotechnology (Reading, Mass.), 1992 - pubmed.ncbi.nlm.nih.gov
… Amino Acid Sequence Bacteriophage M13 / genetics Base Sequence …
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