Parauchenoglanis zebratus sp. nov. is a new species endemic to the Upper Lualaba in the Upper Congo Basin. It is distinguished from all its congeners known from the Congo Basin and adjacent basins by the presence of (1) distinctive dark‐brown or black vertical bars on the lateral side of the body, at least for specimens about ≥120 mm L_S, (2) a broad and triangular humeral process embedded under the skin and (3) a well‐serrated pectoral‐fin spine. Genetic analysis based on mtDNA COI sequences confirmed the genetic distinctiveness (2.8%–13.6% K2P genetic divergence) of P. zebratus sp. nov. from congeners within the Congo and adjacent river basins. The study also revealed additional undocumented diversity within P. ngamensis, P. pantherinus, P. punctatus and P. balayi, indicating the need for further in‐depth alpha‐taxonomic attention to provide more accurate species delimitations for this genus. The discovery of yet another new species endemic to the Upper Lualaba, and this well outside the currently established protected areas, highlights the critical need for further assessments to accurately document the species diversity to guide freshwater conservation prioritisation and biodiversity management in this region.