… a novel protocol for fast and reliable isolation of fungal EVs from … EV isolation from solid
fungal cultures revealed the participation of … These results reveal novel approaches and cellular …

… fungi, including human pathogens. In this protocol, we present two techniques for isolating
… neoformans using Iodixanol gradient revealed that RNAs are co-sedimented with EVs, …

… IMPORTANCE Fungal cells use extracellular vesicles (EVs) … Our results revealed the presence
of typical EVs in protoplast … In agreement with an involvement of EVs in cell wall synthesis, …

… albicans, we evaluated the involvement of EVs in the yeast-to… brasiliensis applied to other
fungi, we isolated EVs from UV-… Moreover, we revealed that the cultures that underwent EVs …

… of phytopathogenic fungi and bacteria. After optimization of the isolation protocol, characterization
of EVs from A. pullulans by transmission electron microscopy (TEM) revealed a typical …

… Further investigations regarding their content and composition revealed that these vesicles
carry dozens of proteins, with functions varying from cell wall assembly and cell signaling to …

… capsulatum vesicles revealed several phospholipids as the … proteins that EVs can deliver
to participate in cell wall repair. … with EVs isolated from different pathogenic fungi. Arrows …

… Fungal EVs participate in the activation of protective immune … Our results suggest that the
methods of isolation of fungal EVs … fungi but reveals a major hurdle for the functional studies of …

… The comparison of all nine fungi revealed that C. … The variability in the EV isolation
methods, mostly based on differential … Alternative protocols of isolation of fungal EVs, including …

… The involvement of vesicular trafficking in fungal FLC resistance has been previously …
regulators of EV biogenesis in fungi. We present data revealing a phenotypic association between …