A novel synthetic-genetic-array–based yeast one-hybrid system for high discovery rate and short processing time
CS Yeh, Z Wang, F Miao, H Ma, CT Kao… - Genome …, 2019 - genome.cshlp.org
Eukaryotic gene expression is often tightly regulated by interactions between transcription
factors (TFs) and their DNA cis targets. Yeast one-hybrid (Y1H) is one of the most
extensively used methods to discover these interactions. We developed a high-throughput
meiosis-directed yeast one-hybrid system using the Magic Markers of the synthetic genetic
array analysis. The system has a transcription factor–DNA interaction discovery rate twice as
high as the conventional diploid-mating approach and a processing time nearly one-tenth of …
factors (TFs) and their DNA cis targets. Yeast one-hybrid (Y1H) is one of the most
extensively used methods to discover these interactions. We developed a high-throughput
meiosis-directed yeast one-hybrid system using the Magic Markers of the synthetic genetic
array analysis. The system has a transcription factor–DNA interaction discovery rate twice as
high as the conventional diploid-mating approach and a processing time nearly one-tenth of …
以上显示的是最相近的搜索结果。 查看全部搜索结果