A qPCR-based protocol to quantify DSB resection
Genome Instability: Methods and Protocols, 2018•Springer
The nucleolytic degradation of the 5′-ending strand of a Double-Strand DNA break (DSB)
is necessary to initiate homologous recombination to correctly repair the break. This process
is called DNA end resection and it is finely regulated to prevent genome rearrangements.
Here, we describe a protocol to quantify DSB resection rate by qPCR, which could be
applied to every organisms whenever the break site and its flanking region sequences are
known.
is necessary to initiate homologous recombination to correctly repair the break. This process
is called DNA end resection and it is finely regulated to prevent genome rearrangements.
Here, we describe a protocol to quantify DSB resection rate by qPCR, which could be
applied to every organisms whenever the break site and its flanking region sequences are
known.
Abstract
The nucleolytic degradation of the 5′-ending strand of a Double-Strand DNA break (DSB) is necessary to initiate homologous recombination to correctly repair the break. This process is called DNA end resection and it is finely regulated to prevent genome rearrangements. Here, we describe a protocol to quantify DSB resection rate by qPCR, which could be applied to every organisms whenever the break site and its flanking region sequences are known.
Springer
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