A reliable protocol for transformation of Catharanthus roseus through Agrobacterium tumefaciens
T Srivastava, S Das, SK Sopory… - Physiology and Molecular …, 2009 - Springer
Proliferation of axillary shoot buds and multiple shoot formation in Catharanthus roseus was
obtained in 96% explants on MS medium (3% sucrose) containing NAA+ BA. 2, 4-D induced
callusing in both, the nodal as well as in leaf segments. Leaf-derived callus was used for
transformation with Agrobacterium tumefaciens LBA4404/pBI-S1. Bacterial cell
concentration, duration of co-cultivation and acetosyringone concentration influenced
transformation efficiency. Under optimal co-cultivation conditions, 98% of the explants …
obtained in 96% explants on MS medium (3% sucrose) containing NAA+ BA. 2, 4-D induced
callusing in both, the nodal as well as in leaf segments. Leaf-derived callus was used for
transformation with Agrobacterium tumefaciens LBA4404/pBI-S1. Bacterial cell
concentration, duration of co-cultivation and acetosyringone concentration influenced
transformation efficiency. Under optimal co-cultivation conditions, 98% of the explants …
A reliable protocol for transformation of Catharanthus roseus through Agrobacterium tumefaciens.
TS Toolika Srivastava, SD Sandip Das, SK Sopory… - 2009 - cabidigitallibrary.org
Proliferation of axillary shoot buds and multiple shoot formation in Catharanthus roseus was
obtained in 96% explants on MS medium (3% sucrose) containing NAA+ BA. 2, 4-D induced
callusing in both, the nodal as well as in leaf segments. Leaf-derived callus was used for
transformation with Agrobacterium tumefaciens LBA4404/pBI-S1. Bacterial cell
concentration, duration of co-cultivation and acetosyringone concentration influenced
transformation efficiency. Under optimal co-cultivation conditions, 98% of the explants …
obtained in 96% explants on MS medium (3% sucrose) containing NAA+ BA. 2, 4-D induced
callusing in both, the nodal as well as in leaf segments. Leaf-derived callus was used for
transformation with Agrobacterium tumefaciens LBA4404/pBI-S1. Bacterial cell
concentration, duration of co-cultivation and acetosyringone concentration influenced
transformation efficiency. Under optimal co-cultivation conditions, 98% of the explants …
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