A single-cell resolution gene expression atlas of the larval zebrafish brain
I Shainer, E Kuehn, E Laurell, M Al Kassar… - Science …, 2023 - science.org
The advent of multimodal brain atlases promises to accelerate progress in neuroscience by
allowing in silico queries of neuron morphology, connectivity, and gene expression. We
used multiplexed fluorescent in situ RNA hybridization chain reaction (HCR) technology to
generate expression maps across the larval zebrafish brain for a growing set of marker
genes. The data were registered to the Max Planck Zebrafish Brain (mapzebrain) atlas, thus
allowing covisualization of gene expression, single-neuron tracings, and expertly curated …
allowing in silico queries of neuron morphology, connectivity, and gene expression. We
used multiplexed fluorescent in situ RNA hybridization chain reaction (HCR) technology to
generate expression maps across the larval zebrafish brain for a growing set of marker
genes. The data were registered to the Max Planck Zebrafish Brain (mapzebrain) atlas, thus
allowing covisualization of gene expression, single-neuron tracings, and expertly curated …
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