Colorectal cancers are characterized by heterogeneity and a hierarchical organization
comprising a population of cancer stem cells (CSCs) responsible for tumor development,
maintenance, and resistance to drugs. A better understanding of CSC properties for their
specific targeting is, therefore, a pre-requisite for effective therapy. However, there is a
paucity of suitable preclinical models for in-depth investigations. Although in vitro two-
dimensional (2D) cancer cell lines provide valuable insights into tumor biology, they do not …