This study aims to analyze the role of academic leadership and work culture on organizational effectiveness as measured by work motivation, job satisfaction, and professional commitment of lecturers. The study population was all permanent lecturers in the five major faculties at Udayana University, namely the Faculty of Cultural Sciences, Medicine, Engineering, Law, and Economics and Business. The sample used was 150 lecturers in the five selected faculties who were determined by quota. The data collection method used was a questionnaire. The analysis technique used in this study is Structural Equation Modeling-Partial Least Square (SEM-PLS). The findings show that academic leadership has a positive and significant effect on work motivation, work motivation has a positive and significant effect on professional commitment, and work motivation fully mediates the effect of academic leadership on lecturers' professional commitment. This research implies that academic leadership needs to be improved in an endeavor to directly increase the work motivation of lecturers. Additionally, academic leadership plays an important role in the activities of the Three Pillars of Higher Education because it increases work motivation which in turn will increase lecturers' job satisfaction.