ear tick, Rhipicephalus appendiculatus (R. appendiculatus), under experimental conditions,
detection of T. parva in ticks and cattle was performed by a quantitative real-time PCR assay.
A calf inoculated with a T. parva mixture became PCR-positive for T. parva infection on day 8
post-inoculation, and subsequently, nymphal ticks were introduced and maintained to feed
on the infected calf for 6 days. Engorged nymphs were collected daily and allowed to molt …