Understanding the behavior of nanomaterials such as nanoparticles into the biological
environment is of crucial importance to develop novel and improved nano-based therapies.
The knowledge at the molecular level of the interactions of nanomaterials with the diseased
target cells and with their biological milieu provides the potential for the rational design of
the next generation of nanoparticles. In this chapter, we highlight the recent advances in the
field of optical microscopy for the study of nanoparticle-cell interactions, with particular …