On 3 April 2017, a M_w 6.5 earthquake struck Moiyabana, Botswana, nucleating at >20 km focal depth within the Paleoproterozoic Limpopo‐Shashe orogenic belt separating the Archean Zimbabwe and Kaapvaal Cratons. We investigate the lithospheric structures associated with this earthquake using high‐resolution aeromagnetic and gravity data integrated with Differential Interferometric Synthetic Aperture Radar (DInSAR) analysis. Here we present the first results that provide insights into the tectonic framework of the earthquake. The ruptured fault trace delineated by DInSAR aligns with a distinct NW striking and NE dipping magnetic lineament within the Precambrian basement. The fault plane solution and numerical modeling indicate that the cause of the earthquake was 1.8 m displacement along a NW striking and NE dipping normal fault, rupturing at 21–24 km depth. We suggest that this seismic event was due to extensional reactivation of a crustal‐scale Precambrian thrust splay within the Limpopo‐Shashe orogenic belt.