Affordable infectious pathogen detection using a dual-mode biosensor integrating exonuclease III-assisted target recycling amplification with high-throughput 96-well …

H Moustakim, A Amine, H Mohammadi - Enzyme and Microbial Technology, 2025 - Elsevier
The ongoing challenge of infectious pathogens highlights the need for accurate and
accessible methods to discern their genetic signatures, especially in resource-limited
settings. In response to this crucial requirement, we introduce an affordable large-scale
screening platform for infectious pathogen detection, using Hepatitis B virus (HBV) as a
fundamental model. This proposed biosensor integrates an exonuclease III-assisted target
recycling amplification strategy within a high-throughput 96-well microplate format. The HBV …
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