The Linecheek tank goby Glossogobius sparsipapillus was originally described by Akihito & Meguro [1]. It is one of three species of the genus Glossogobius recorded from brackish to freshwater in the Mekong Delta [2]-[4]. This goby displays isometric growth in Soc Trang, downstream of Hau river [5]. The species is a multiple spawner based on testis and ovarian histological description [6],[7] and a carnivore based on the relative gut length analysis [8]. Some morphometric and meristic characteristics of G. sparsipapillus caught from different places along the coastline in the Mekong Delta are reported by Nguyen et al.(2020)[4]. However, these authors did not verify whether fish sex can be determined based on morphometric data and their fish collection was collected from some estuarine and coastal regions in the Mekong Delta. This study aims to test whether this goby’s allometry change with gender, fish size, season, and sampling site variables based on a new collection of Glossogobius sparsipapillus from Hau River in Can Tho and Soc Trang provinces.