Análises das isoformas de heparanase e da catepsina B em plasma de pacientes com carcinomas gastrointestinais: Estudo transversal analítico
CM Melo, CST Origassa, TR Theodoro… - Sao Paulo Medical …, 2014 - SciELO Brasil
Sao Paulo Medical Journal, 2014•SciELO Brasil
CONTEXT AND OBJECTIVE: Heparanase-1 degrades heparan sulfate and has been
correlated with tumor progression. Although the isoform heparanase-2 has no catalytic
activity, it seems to be important for modulating heparanase-1 activity. Cathepsin B is a
proteinase involved in tumor metastasis. The aim of this study was to analyze heparanase
isoform expression and cathepsin B activity in plasma samples from patients with
gastrointestinal carcinomas, compared with healthy individuals (control group). DESIGN …
correlated with tumor progression. Although the isoform heparanase-2 has no catalytic
activity, it seems to be important for modulating heparanase-1 activity. Cathepsin B is a
proteinase involved in tumor metastasis. The aim of this study was to analyze heparanase
isoform expression and cathepsin B activity in plasma samples from patients with
gastrointestinal carcinomas, compared with healthy individuals (control group). DESIGN …
CONTEXT AND OBJECTIVE
Heparanase-1 degrades heparan sulfate and has been correlated with tumor progression. Although the isoform heparanase-2 has no catalytic activity, it seems to be important for modulating heparanase-1 activity. Cathepsin B is a proteinase involved in tumor metastasis. The aim of this study was to analyze heparanase isoform expression and cathepsin B activity in plasma samples from patients with gastrointestinal carcinomas, compared with healthy individuals (control group).
DESIGN AND SETTING
This was an analytical cross-sectional study. Peripheral blood samples were collected at a Brazilian public hospital, from 21 patients with histopathological diagnoses of gastrointestinal carcinomas and from 43 healthy individuals. The analyses were performed in two Brazilian medical schools.
METHODS
Heparanase isoforms were identified and quantified in plasma samples by means of Western blot. The enzymatic activities of heparanase-1 and cathepsin B were also measured.
RESULTS
The results demonstrated that the expression of both heparanase isoforms was significantly greater in plasma samples from gastrointestinal carcinoma patients, compared with the control group. Logistic regression analysis showed that increased heparanase-1 and heparanase-2 expression was exclusively dependent on the tumor. There was a significant increase in heparanase-1 and cathepsin B activity in the patients' plasma.
CONCLUSION
Overexpression of heparanase-1 and heparanase-2, along with increased heparanase-1 and cathepsin B activity in plasma, is associated with the diagnosis of gastrointestinal carcinoma. These findings provide support for using non-invasive assays (plasma samples) as an auxiliary method for diagnosing gastrointestinal tumors.
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