An affinity-based aqueous two-phase mixed micellar system and its purification of Yeast 3′, 5′-bisphosphate nucleotidase
Y Liu, MH Sun, SK Shao, G Deng - Journal of Chromatography B, 2017 - Elsevier
Aqueous two-phase micellar system (ATPMS), as an alternative liquid–liquid extraction
technique, has been extensively exploited for the precise separation or large-scale
concentration of biomolecules. In this article, a novel affinity-based ATPMS composed of
mixed micelles was constructed by introducing a Copper-chelated Triton X-114 (TX-Cu (II))
into an aqueous solution of hydrophobically modified ethylene oxide polymer (HM-EO).
Phase diagram of the HM-EO/TX-Cu (II) system was measured, and the partitioning behavior …
technique, has been extensively exploited for the precise separation or large-scale
concentration of biomolecules. In this article, a novel affinity-based ATPMS composed of
mixed micelles was constructed by introducing a Copper-chelated Triton X-114 (TX-Cu (II))
into an aqueous solution of hydrophobically modified ethylene oxide polymer (HM-EO).
Phase diagram of the HM-EO/TX-Cu (II) system was measured, and the partitioning behavior …
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