We are reporting you an effective DNA vaccine for FMD virus, and it was prepared using mannosylated chitosan (MC) nanoparticles to form the FMDV-pVAC-VP1-OmpA complex particles. These particles were characterized for their physical properties like morphology, size and charge prior evaluating the actual vaccine delivery effect of MC-nanoparticles. The immunological evaluation indicated that the 20 μg of DNA vaccine complexed with MC nano-particles was found optimum in inducing the immune response in G pigs as measured by FMDV specific neutralising antibodies and Th1/Th2 responses using micro SNT and ELISA, respectively.