An exploratory investigation of the CSF metabolic profile of HIV in a South African paediatric cohort using GCxGC-TOF/MS
Metabolomics, 2024•Springer
Introduction Because cerebrospinal fluid (CSF) samples are difficult to obtain for paediatric
HIV, few studies have attempted to profile neurometabolic dysregulation. Aim and objective
The aim of this exploratory study was to profile the neurometabolic state of CSF from a South
African paediatric cohort using GCxGC-TOF/MS. The study included 54 paediatric cases (<
12 years), 42 HIV-negative controls and 12 HIV-positive individuals. Results The results
revealed distinct metabolic alterations in the HIV-infected cohort. In the PLS-DA model, 18 …
HIV, few studies have attempted to profile neurometabolic dysregulation. Aim and objective
The aim of this exploratory study was to profile the neurometabolic state of CSF from a South
African paediatric cohort using GCxGC-TOF/MS. The study included 54 paediatric cases (<
12 years), 42 HIV-negative controls and 12 HIV-positive individuals. Results The results
revealed distinct metabolic alterations in the HIV-infected cohort. In the PLS-DA model, 18 …
Introduction
Because cerebrospinal fluid (CSF) samples are difficult to obtain for paediatric HIV, few studies have attempted to profile neurometabolic dysregulation.
Aim and objective
The aim of this exploratory study was to profile the neurometabolic state of CSF from a South African paediatric cohort using GCxGC-TOF/MS. The study included 54 paediatric cases (< 12 years), 42 HIV-negative controls and 12 HIV-positive individuals.
Results
The results revealed distinct metabolic alterations in the HIV-infected cohort. In the PLS-DA model, 18 metabolites significantly discriminated between HIV-infected and control groups. In addition, fold-change analysis, Mann–Whitney U tests, and effect size measurements verified these findings. Notably, lactose, myo-inositol, and glycerol, although not significant by p-value alone, demonstrated practical significance based on the effect size.
Conclusions
This study provided valuable insights on the impact of HIV on metabolic pathways, including damage to the gut and blood–brain barrier, disruption of bioenergetics processes, gliosis, and a potential marker for antiretroviral therapy. Nevertheless, the study recognized certain constraints, notably a limited sample size and the absence of a validation cohort. Despite these limitations, the rarity of the study’s focus on paediatric HIV research underscores the significance and unique contributions of its findings.
Springer
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