Analysis of the cytokine profile in spleen cells from dogs naturally infected by Leishmania chagasi
RS Lage, GC Oliveira, SU Busek, LL Guerra… - Veterinary immunology …, 2007 - Elsevier
Recent studies suggest that asymptomatic dogs infected with canine visceral leishmaniasis
(CVL) develop a Th1 immunological profile whilst oligosymptomatic and symptomatic CVL-
infected animals present a Th2 profile. In the present study, an RT-PCR method has been
standardised and employed to evaluate the frequency and the semi-quantitative level of
expression of the cytokines IL-4, IL-10, IL-12, INF-γ and TNF-α in splenocytes of 30 dogs
naturally infected with Leishmania chagasi and of 7 non-infected dogs (NID). An increase in …
(CVL) develop a Th1 immunological profile whilst oligosymptomatic and symptomatic CVL-
infected animals present a Th2 profile. In the present study, an RT-PCR method has been
standardised and employed to evaluate the frequency and the semi-quantitative level of
expression of the cytokines IL-4, IL-10, IL-12, INF-γ and TNF-α in splenocytes of 30 dogs
naturally infected with Leishmania chagasi and of 7 non-infected dogs (NID). An increase in …