Lantana camara Linn is a family of Verbenaceae which grows wild and is widespread in various both tropical and sub-tropical countries. Isolation essential oil of the L. camara leaves extracted by hydrodistillation and were analyzed by Gas Chromatography-Mass Spectrometry (GC-MS). The results of GC-MS analysis show isocaryophyllene (14.39%), Ï-cymene (8.17%), β-cubebene (7.8%), α-pinene (7.64%), and β-elemene (5.51%) as the main compound. The cytotoxic activity of the isolated essential oil was highly toxic both to Artemia salina shrimp larvae (LC 50 15.92 µg/mL) and to breast cancer cells T-47D (IC 50 10.67 µM).