Background: The goal of this study was to investigate the anthelmintic activity of a methanol extract of Zingiber roseum rhizome (ZRR) in an experimental model, followed by an in silico molecular docking study and an ADMET analysis. Methods: A worm called Pheretima posthuma was used to test the anthelmintic activity. Then, molecular docking study was performed of identified compounds of Z. roseum rhizome from HPLC namelycatechin hydrate, epicatechin, trans-ferulic acid, rosmarinic acid, quercetin, myricetin, trans-cinnamic acid having maximum activity against β-TUBULIN enzyme by using CB-Dock protein-ligand docking server. Additionally, DruLiTo software was used to investigate drug-like predictions, and ADME/T profiles were examined using Swiss ADME Analysis and Molinspiration Cheminformatics software.

Results: ZRR contained alkaloids, flavonoids, tannins, and saponins, according to preliminary phytochemical analysis. On an adult worm Pheretima posthuma, ZRR exhibited dose-dependent and statistically significant anthelmintic activityThe best concentration of ZRR for anthelmintic activity on Pheretima posthuma was compared to the reference standard Albendazole (2.5 & 5.0 mg/ml). Our molecular docking study, on the other hand, reveals that the phenolic compounds (except trans-cinnamic acid) have strong binding affinities (ranging from-6.4 to-8.5 KCal/mol) to β-tubulin. These phytocompounds from natural sources could be used to develop a reliable medication or to aid in lead identification using modern techniques. Conclusion: The current study's findings confirmed Zingiber roseum rhizome extract's potential anthelmintic activity, and all compounds (except trans-cinnamic acid) were found to be effective in computer-aided drug design models.