The present study evaluated the anti-lipidemic activity of water leaf extract of Desmodium velutinum on wistar albino rats that were fed with high fat diet. The results revealed that group 2 rats (treated with 10mg/ml of high fat diet) had the highest level of low density lipoprotein (LDL) cholesterol but the increase was not significant (p> 0.05) when compared with that of control group rats (group 1) that were fed with only normal feed. Groups 3 and 4 rats administered with 10mg/ml of high fat diet+ 5mg/kg of standard drug atorvastatin and 10mg/ml of high fat diet+ 5mg/kg of water extract of Desmodium velutinum respectively non-significantly decreased (p> 0.05) the LDL cholesterol of the rats when compared with that of control group 1 (fed with only normal feed) and group2 (treated with 10mg/ml of high fat diet). Group 4 (rats treated with 10mg/ml of high fat diet+ 5mg/kg of Desmodium velutinum) significantly increased (p< 0.05) in high density lipoprotein (HDL) cholesterol concentration when compared to groups 3 and 2 rats. Total cholesterol concentration of group 2 non-significantly increased (p> 0.05) when compared with that of group 1 but total-cholesterol concentration of group 3 rats significantly decreased (p< 0.05) when compared to that of groups 1 and 2 rats. Triacylglycerol concentration of group 2 rats increased significantly (p< 0.05) when compared with that of group 1 rats. Groups 3 and 4 rats significantly decreased in triacylglycerol concentrations of the rats when compared with that of groups 1 and 2 rats. Hyperlipidemic.