Bergenia ligulata Wall., family Saxifragaceae, is an Indian folk medicine used for a variety of pharmacological effects. In this study, evidence is provided in animal model to demonstrate the role of aqueous as well as 50% ethanolic extract of B. ligulata in inflammation and as antibacterial agent. Oral administration of the extract at a dose level of 1 gm/kg bw showed anti-inflammatory and free radical scavenging activity as evaluated using pharmacological and biochemical parameters. The effect was studied on biochemical parameters reportedly perturbed in inflammation. While the extract treatment could alleviate the level of succinate dehydrogenase and xanthine oxidase, which increase in inflammation, the level of superoxide dismutase increased following the treatment with the extract as well as the diclofenac. Role of oxygen free radicals/peroxides was evaluated by measuring lipid peroxidation and glutathione. Treatment with the extract could significantly decrease the enhanced level of lipid peroxidation in inflammation, and increased the level of glutathione. Further, the antibacterial activity of various fractions was tested in vitro using cultures of Escherichia coli, Baccillus subtilis, and S. aureus, and the fractions were found to be antibacterial. The antifungal activity was also tested using the culture of Saccharomyces. However, the drug was ineffective in inhibiting fungal growth. Results provide evidence suggesting the anti-inflammatory as well as the antibacterial role of B. ligulata, thus implicating the plant extract in treatment against the bacterial infection and inflammation.