The methanolic extract of leaf, flower and stem of Leonotis nepetifolia was analyzed for antibacterial activity against eight pathogenic bacterial isolates viz., Escherichia coli, Pseudomonas aeruginosa, Shigella dysenteriae, Shigella flexneri, Vibrio cholerae, Bacillus subtilis, Micrococcus luteus and Saphylococcus aureus. The extract was also subjected to qualitative tests for alkaloids, tannins, terpenoids, steroids, saponins and flavonoids. The methanolic extract exhibited antibacterial activity against all the bacteria tested indicating the presence of broad spectrum antibacterial compounds in the plant parts. Gram positive bacteria were found to be more susceptible than Gram negative bacteria. The leaf and flower extract of the plant showed higher activity than the stem. Phytochemical examination indicated the presence of alkaloids, saponins, tannins and terpenoids.