Karangploso, District Malang is known as a milk producing area and is a suitable place for the development of dairy cattle business. Mastitis is one of the most common problems found in dairy farming in Indonesia that has a negative impact on the milk production economy. The study was conducted to examine the incidence of antibiotic resistance as an attempt to appropriate treatment for the treatment of mastitis in dairy cows. A total of 85 positive samples are Staphylococcus aureus and Streptococcus sp from farms in the KUD Karangploso region. Based on the sensitivity test against various antibiotics it is known that Staphylococcus aureus has been resistant to Penicillin (100%), Tetracycline (48.23%), Erythromycin (44.70%), Gentamicin (2.35%), and Ampicillin (1.18% ). For Streptococcus sp has been resistant to antibiotic Penicillin (98.82%), Erythromycin (94.18%), Tetracycline (84.70%), Gentamicin (11.76%), Ampicillin (5.88%), and Cefalexin (3.53%). Key words: Subclinical mastitis, California Mastitis Test (CMT), Staphylococcus aureus and, Streptococcus sp, antibiotics resistant