Nowadays, nanotechnology is receiving worldwide recognition due to being its applications in various fields. Medium temperature and eco-friendly methods have shown great potential among nanoparticle synthesis. Many reducing agents are available for the synthesis of ZnO nanoparticles; here we presented a simple solution method for the development of nano crystalline Zinc Oxide particles. Zinc acetate dihydrate was used as a starting material and synthesized particles were characterized by UV-Visible, FT IR, SEM, and EDAX analysis. Characterization studies revealed hexagonal nano crystalline Zinc Oxide particles with excellent morphological properties. The synthesized nanoparticles exhibit excellent antimicrobial activities against gram-positive and gram-negative bacteria.