This study was carried out to assess alpha-fetoprotein, albumin, CD4+ and some liver enzymes in HIV infected adult on ART in NAUTH Nnewi, South Eastern Nigeria. A total of 97 participants who were aged between 18 and 60 years attending the voluntary counseling and testing unit (VCT) and antiretroviral therapy unit (ART) of Nnamdi Azikiwe University Teaching Hospital (NAUTH), Nnewi were randomly recruited for the study and grouped based on WHO criteria for HIV staging. Six millilitres (6mls) of blood sample were collected from each of the participants in each group and dispensed into EDTA and plain containers in appropriate volumes for the determination of the alpha fetoprotein (AFP), albumin (ALB), alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) and CD4+ count using standard laboratory methods. Results showed statistically significant increases in the mean serum albumin level and CD4+ count in HIV/AIDS participants on ART than in those not on ART but significantly lower in both HIV/AIDS subjects on ART and those not on ART compared with control subjects respectively (p< 0.05). AFP, AST and ALP activities remained the same when compared between the groups (p> 0.05). Also, ALT activity did not differ significant in HIV/AIDS participants on ART compared with HIV/AIDS participants not on ART (p> 0.05) but were significantly higher than in control subjects respectively (p< 0.05). Therefore, this study has shown no hepatocellular carcinoma or hepatotoxicity emanating from antiretroviral drugs use in HIV/AIDS patients rather it revealed an immune system recovery in HIV/AIDS subjects on ART.