AtRTPrimer: database for Arabidopsis genome-wide homogeneous and specific RT-PCR primer-pairs
S Han, D Kim - BMC bioinformatics, 2006 - Springer
Background Primer design is a critical step in all types of RT-PCR methods to ensure
specificity and efficiency of a target amplicon. However, most traditional primer design
programs suggest primers on a single template of limited genetic complexity. To provide
researchers with a sufficient number of pre-designed specific RT-PCR primer pairs for whole
genes in Arabidopsis, we aimed to construct a genome-wide primer-pair database.
Description We considered the homogeneous physical and chemical properties of each …
specificity and efficiency of a target amplicon. However, most traditional primer design
programs suggest primers on a single template of limited genetic complexity. To provide
researchers with a sufficient number of pre-designed specific RT-PCR primer pairs for whole
genes in Arabidopsis, we aimed to construct a genome-wide primer-pair database.
Description We considered the homogeneous physical and chemical properties of each …
[引用][C] AtRTPrimer: database for Arabidopsis genome-wide homogeneous and specific RT-PCR primer-pairs
K Dongsup, H Sangjo - 2006
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