Microbial alkaline proteases are among the important hydrolytic enzyme and have been used extensively since the advent of enzymology. Bactrial extracellular alkaline proteases are of great importance due to its wide spectrum applications in detergent industries, bioremediation, food industries, and leather processing and bio-film degradradation. From the various niches eighteen isolates were screened for alkaline protease production, out of which six isolates showed efficient enzyme production. The ratio of zone diameter to colony diameter of six isolates ranged from 2.11 to 7.33. Out of six two isolates B. pumilus p1 and S. auricularis p18 showed significant enzyme activity. Optimization of the incubation period, pH and temperature conditions for enzyme production were determined and were found to be 72hrs, 8.0-9.0 and 450C respectively. Optimization of nitrogen sources for enzyme production were determined and was found to be 0.9% for peptone and 0.5% for yeast extract for both the isolates. Enzyme activity was assayed using tyrosine-casein method. The purified enzyme preparations of B. pucilum and S. auricularis having enzyme activity 0.065 U/ml and 0.038 U/ml respectively were also excellent in dehairing and depilating of raw leather, recovery of silver from X-ray photographic films and bacterial biofilm degradation.