The most important ingredient in Bayesian analysis is prior or prior distribution. A new prior determination method was developed under the framework of parametric empirical Bayes using bootstrap technique. By way of example, Bayesian estimations of the parameters of a normal distribution with unknown mean and unknown variance conditions were considered, as well as its application in comparing the means of two independent normal samples with several scenarios. A Monte Carlo study was conducted to illustrate the proposed procedure in estimation and hypothesis testing. Results from Monte Carlo studies showed that the bootstrap prior proposed is more efficient than the existing method for determining priors and also better than the frequentist methods reviewed.