Laboratory experiment was conducted to determine the bio-efficacy of different strains of entomopathogenic fungi viz., Beauveria bassiana, Metarhizium anisopliae and Nomuraea rileyi and entomopathogenic bacteria viz., Bacillus thuringiensis against fall armyworm (FAW) Spodoptera frugiperda (JE Smith)(Lepidoptera: Noctuidae) under laboratory condition. Among different entomopathogenic fungi evaluated, AAU strain of M. anisopliae@ 2x109 conidia/ml showed the highest larval mortality ie, 57.56% and 50.63% against 2nd and 3rd instar larvae respectively. The same strain registered the larval mortality of 55.50% and 48.45% against 2nd and 3rd instar larvae respectively@ 2 x 108 conidia/ml. In case of entomopathogenic bacteria, AAU strain of Bacillus thuringiensis showed 64.65% and 59.01% larval mortality against 2nd and 3rd instar larvae respectively when spore-crystal mixture was tested@ 3 ml. The present study emphasizes the significant efficacy of native strains of entomopathogenic fungi and bacteria as compared to non-native strains against invasive pest S. frugiperda.