[PDF][PDF] Biochemical Identification of a Mutated Human Melanoma Antigen Recognized by CD4 T Cells

R embert Pieper, MI Nishimura, G Gupta… - The Journal of …, 1999 - academia.edu
CD4 T cells play a critical role in generating and maintaining immune responses against
pathogens and alloantigens, and evidence suggests an important role for them in antitumor
immunity as well. Although major histocompatibility complex class II–restricted human CD4
T cells with specific antitumor reactivities have been described, no standard method exists
for cloning the recognized tumor-associated antigen (Ag). In this study, biochemical protein
purification methods were used in conjunction with novel mass spectrometry sequencing …

Biochemical Identification of a Mutated Human Melanoma Antigen Recognized by CD4+ T Cells

R Pieper, RE Christian, MI Gonzales… - The Journal of …, 1999 - rupress.org
CD4+ T cells play a critical role in generating and maintaining immune responses against
pathogens and alloantigens, and evidence suggests an important role for them in antitumor
immunity as well. Although major histocompatibility complex class II–restricted human CD4+
T cells with specific antitumor reactivities have been described, no standard method exists
for cloning the recognized tumor-associated antigen (Ag). In this study, biochemical protein
purification methods were used in conjunction with novel mass spectrometry sequencing …
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