studied. When stored at 4° C or higher, the free fatty acid content in the wet biomass
increased from a trace to 62.0% by day 4. Using two-step catalytic conversion, algae oil with
a high free fatty acid content was converted to biodiesel by pre-esterification and
transesterification. The conversion rate of triacylglycerols reached 100% under the methanol
to oil molar ratio of 12: 1 during catalysis with 2% potassium hydroxide at 65° C for 30min …