CRISPR/Cas9-mediated multi-allelic gene targeting in sugarcane confers herbicide tolerance
Sugarcane is the source of 80% of the sugar and 26% of the bioethanol produced globally.
However, its complex, highly polyploid genome (2 n= 100–120) impedes crop improvement.
Here, we report efficient and reproducible gene targeting (GT) in sugarcane, enabling
precise co-editing of multiple alleles via template-mediated and homology-directed repair
(HDR) of DNA double strand breaks induced by the programmable nuclease CRISPR/Cas9.
The evaluation of 146 independently transformed plants from five independent experiments …
However, its complex, highly polyploid genome (2 n= 100–120) impedes crop improvement.
Here, we report efficient and reproducible gene targeting (GT) in sugarcane, enabling
precise co-editing of multiple alleles via template-mediated and homology-directed repair
(HDR) of DNA double strand breaks induced by the programmable nuclease CRISPR/Cas9.
The evaluation of 146 independently transformed plants from five independent experiments …
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