Calcium-mediated DNA adsorption to yeast cells and kinetics of cell transformation by electroporation
E Neumann, S Kakorin, I Tsoneva, B Nikolova… - Biophysical journal, 1996 - cell.com
Detailed kinetic data suggest that the direct transfer of plasmid DNA (YEp 351, 5.6 kbp,
supercoiled, Mr approximately 3.5 x 10 (6)) by membrane electroporation of yeast cells
(Saccharomyces cerevisiae, strain AH 215) is mainly due to electrodiffusive processes. The
rate-limiting step for the cell transformation, however, is a bimolecular DNA-binding
interaction in the cell interior. Both the adsorption of DNA, directly measured with [32P] dCTP
DNA, and the number of transformants are collinearly enhanced with increasing total …
supercoiled, Mr approximately 3.5 x 10 (6)) by membrane electroporation of yeast cells
(Saccharomyces cerevisiae, strain AH 215) is mainly due to electrodiffusive processes. The
rate-limiting step for the cell transformation, however, is a bimolecular DNA-binding
interaction in the cell interior. Both the adsorption of DNA, directly measured with [32P] dCTP
DNA, and the number of transformants are collinearly enhanced with increasing total …
[PDF][PDF] Calcium-Mediated DNA Adsorption to Yeast Cells and Kinetics of Cell Transformation by Electroporation
BJV August1996 - core.ac.uk
Detailed kinetic data suggest that the direct transfer of plasmid DNA (YEp 351, 5.6 kbp,
supercoiled, M, 3.5 x 1 o6) by membrane electroporation of yeast cells (Saccharomyces
cerevisiae, strain AH 215) is mainly dueto electrodiffusive processes. The rate-limiting step
for the cell transformation, however, is a bimolecular DNA-binding interaction in the cell
interior. Both the adsorption of DNA, directly measured with [32P] dCTP DNA, and the
number of transformants are collinearly enhanced with increasing total concentrations [DJ …
supercoiled, M, 3.5 x 1 o6) by membrane electroporation of yeast cells (Saccharomyces
cerevisiae, strain AH 215) is mainly dueto electrodiffusive processes. The rate-limiting step
for the cell transformation, however, is a bimolecular DNA-binding interaction in the cell
interior. Both the adsorption of DNA, directly measured with [32P] dCTP DNA, and the
number of transformants are collinearly enhanced with increasing total concentrations [DJ …
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