Developing computational analysis of time-lapse imaging of calcium events in astrocytes is
a challenging task in neuroscience. Here we report the implementation of an algorithm that
solves this task. After noise reduction with the block-matching and 3D filtering (BM3D)
algorithm, calcium activity is identified as fluorescence elevation above the baseline level.
Individual events are detected by sliding window approach applied to the variation of pixel
intensity relative to the baseline level. The maximal projection and duration of astrocytic …