Characterization in vitro and in vivo of the putative multigene 4-coumarate: CoA ligase network in Arabidopsis: syringyl lignin and sinapate/sinapyl alcohol derivative …
MA Costa, DL Bedgar, SGA Moinuddin, KW Kim… - Phytochemistry, 2005 - Elsevier
A recent in silico analysis revealed that the Arabidopsis genome has 14 genes annotated as
putative 4-coumarate: CoA ligase isoforms or homologues. Of these, 11 were selected for
detailed functional analysis in vitro, using all known possible phenylpropanoid pathway
intermediates (p-coumaric, caffeic, ferulic, 5-hydroxyferulic and sinapic acids), as well as
cinnamic acid. Of the 11 recombinant proteins so obtained, four were catalytically active in
vitro, with fairly broad substrate specificities, confirming that the 4CL gene family in …
putative 4-coumarate: CoA ligase isoforms or homologues. Of these, 11 were selected for
detailed functional analysis in vitro, using all known possible phenylpropanoid pathway
intermediates (p-coumaric, caffeic, ferulic, 5-hydroxyferulic and sinapic acids), as well as
cinnamic acid. Of the 11 recombinant proteins so obtained, four were catalytically active in
vitro, with fairly broad substrate specificities, confirming that the 4CL gene family in …
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