Characterization of Na/K-ATPase in Macrobrachium rosenbergii and the effects of changing salinity on enzymatic activity
MN Wilder, M Atmomarsono, TTT Hien, TQ Phu… - … and Physiology Part A …, 2000 - Elsevier
A ouabain-sensitive Na/K-ATPase kinetic assay system based on the hydrolysis of ATP and
the oxidation of NADH was adapted in order to characterize enzymatic activity in gills and
examine the effects of changing salinity in Macrobrachium rosenbergii. Maximum inhibition
by ouabain occurred at a concentration of 1.4 mM, and the Km of the reaction was 0.2 mM. In
a first experiment, animals were acclimated to freshwater, 1/3 seawater, 2/3 seawater and
full seawater for up to 1 week. Na/K-ATPase activity in front gills was 1.62±0.19 μmol …
the oxidation of NADH was adapted in order to characterize enzymatic activity in gills and
examine the effects of changing salinity in Macrobrachium rosenbergii. Maximum inhibition
by ouabain occurred at a concentration of 1.4 mM, and the Km of the reaction was 0.2 mM. In
a first experiment, animals were acclimated to freshwater, 1/3 seawater, 2/3 seawater and
full seawater for up to 1 week. Na/K-ATPase activity in front gills was 1.62±0.19 μmol …