Characterization of antigen-specific B cells using nominal antigen-coated flow-beads

N Degauque, AE Ngono, A Akl, M Lepetit… - PLoS …, 2013 - journals.plos.org
In order to characterize the reactivity of B cells against nominal antigens, a method based on
the coupling of antigens onto the surface of fluorescent core polystyrene beads was
developed. We first demonstrate that murine B cells with a human MOG-specific BCR are
able to interact with MOG-coated beads and do not recognize beads coated with human
albumin or pp65. B cells purified from human healthy volunteer blood or immunized
individuals were tested for their ability to interact with various nominal antigens, including …

Correction: Characterization of Antigen-Specific B Cells Using Nominal Antigen-Coated Flow-Beads

PLOS ONE Staff - Plos one, 2014 - journals.plos.org
Correction: Characterization of Antigen-Specific B Cells Using Nominal Antigen-Coated
Flow-Beads … (2013) Characterization of Antigen-Specific B Cells Using Nominal Antigen-Coated
Flow-Beads. PLoS ONE 8(12): e84273. doi:10.1371/journal.pone.0084273 … This is an
open-access article distributed under the terms of the Creative Commons Attribution License,
which permits unrestricted use, distribution, and reproduction in any medium, provided the
original author and source are credited. …
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