Background: Recent advances in high-throughput single-cell sequencing technologies have
led to their increasingly widespread adoption for clinical applications. However, challenges
associated with tissue viability, cell yield, and delayed time-to-capture have created unique
obstacles for data processing. Chronic wounds, in particular, represent some of the most
difficult target specimens, due to the significant amount of fibrinous debris, extracellular
matrix components, and non-viable cells inherent in tissue routinely obtained from …