Tunisian wild edible hawthorn fruits (Crataegus spp.) may be used in many human fields due to their macro-or/and micronutriments such as flavonoids but limited information is available about the characterization of these compounds in hawthorn fruits. However, the aim of this work was to characterize the phytochemical composition of both Crataegus species. The results of liquid chromatography LC-ESI/MS show the identification of nine flavonoids glucosides for the studied Crataegus extracts such as: isoquercitin, quercitin-digalactoside, quercitin-diglucoside, quercitin-3-O-galactoside, Kaempherol-3-galactoside, Kaempherol-3-glucoside, luteolin-7-O-rutinoside, apigenin-7-O-glucoside, catechin and epicatechin. MTT test show that MCF-7 cell line present different degrees of sensitivity to the both hawthorn fruits species. C. monogyna specie present very important effectiveness against the tumor cell line MCF-7while C. azarolus is moderately active against the tested line