Experiment was conducted to evaluate the effect of increasing fibrolytic enzyme level on nutrient composition and digestion kinetics of urea treated wheat straw (WS). Wheat straw was treated with 4% urea and 6% molasses and was ensiled with 0 (E0), 1 (E1), 2 (E2) and 3 (E3) g of enzyme/Kg of dry matter (DM). Enzyme mixture was dissolved in water and sprayed on WS. Then after an hour of enzyme treatment, molasses and urea were dissolved in water and sprayed on enzyme-treated WS. Wheat straw was ensiled in 36 laboratory silos under Completely Randomized Design for twenty one days. Application of enzymes on ensiled WS did not affect (P> 0.05) DM, crude protein, true protein, neutral detergent fiber (NDF) and acid detergent fiber (ADF) contents. Enzyme treatment did not affect (P> 0.05) the pH of the ensiled WS. Digestion kinetics of DM, NDF and ADF also remained unaltered (P> 0.05) across all the treatments. On the basis of results it is concluded that enzyme did not affect the nutrient profile of WS because of alkaline pH due to rapid production of ammonia in the silo.