Chromatographic Isolation of Methionine-containing Peptides for Gel-free Proteome Analysis: Identification Of More Than 800 Escherichia Coli Proteins* S
K Gevaert, J Van Damme, M Goethals… - Molecular & cellular …, 2002 - ASBMB
A novel gel-free proteomic technology was used to identify more than 800 proteins from 50
million Escherichia coli K12 cells in a single analysis. A peptide mixture is first obtained from
a total unfractionated cell lysate, and only the methionine-containing peptides are isolated
and identified by mass spectrometry and database searching. The sorting procedure is
based on the concept of diagonal chromatography but adapted for highly complex mixtures.
Statistical analysis predicts that we have identified more than 40% of the expressed …
million Escherichia coli K12 cells in a single analysis. A peptide mixture is first obtained from
a total unfractionated cell lysate, and only the methionine-containing peptides are isolated
and identified by mass spectrometry and database searching. The sorting procedure is
based on the concept of diagonal chromatography but adapted for highly complex mixtures.
Statistical analysis predicts that we have identified more than 40% of the expressed …
以上显示的是最相近的搜索结果。 查看全部搜索结果