Cloning and expression of new microRNAs from zebrafish
WP Kloosterman, FA Steiner, E Berezikov… - Nucleic Acids …, 2006 - academic.oup.com
MicroRNAs (miRNAs) play an important role in development and regulate the expression of
many animal genes by post-transcriptional gene silencing. Here we describe the cloning
and expression of new miRNAs from zebrafish. By high-throughput sequencing of small-
RNA cDNA libraries from 5-day-old zebrafish larvae and adult zebrafish brain we found 139
known miRNAs and 66 new miRNAs. For 65 known miRNAs and for 11 new miRNAs we
also cloned the miRNA star sequence. We analyzed the temporal and spatial expression …
many animal genes by post-transcriptional gene silencing. Here we describe the cloning
and expression of new miRNAs from zebrafish. By high-throughput sequencing of small-
RNA cDNA libraries from 5-day-old zebrafish larvae and adult zebrafish brain we found 139
known miRNAs and 66 new miRNAs. For 65 known miRNAs and for 11 new miRNAs we
also cloned the miRNA star sequence. We analyzed the temporal and spatial expression …
[PDF][PDF] Cloning and expression of new microRNAs from Zebrafish
FA Steiner, E de Bruijn, E Cuppen - 2006 - Citeseer
MicroRNAs (miRNAs) play an important role in development and regulate the expression of
many animal genes by post-transcriptional gene silencing. Here we describe the cloning
and expression of new miRNAs from zebrafish. By high-throughput sequencing of small-
RNA cDNA libraries from 5-day-old zebrafish larvae and adult zebrafish brain we found 139
known miRNAs and 66 new miRNAs. For 65 known miRNAs and for 11 new miRNAs we
also cloned the miRNA star sequence. We analyzed the temporal and spatial expression …
many animal genes by post-transcriptional gene silencing. Here we describe the cloning
and expression of new miRNAs from zebrafish. By high-throughput sequencing of small-
RNA cDNA libraries from 5-day-old zebrafish larvae and adult zebrafish brain we found 139
known miRNAs and 66 new miRNAs. For 65 known miRNAs and for 11 new miRNAs we
also cloned the miRNA star sequence. We analyzed the temporal and spatial expression …