In recent years, two-photon calcium imaging has become a standard tool to probe the
function of neural circuits and to study computations in neuronal populations. However, the
acquired signal is only an indirect measurement of neural activity due to the comparatively
slow dynamics of fluorescent calcium indicators. Different algorithms for estimating spike
rates from noisy calcium measurements have been proposed in the past, but it is an open
question how far performance can be improved. Here, we report the results of the …

In recent years, two-photon calcium imaging has become a standard tool to probe the
function of neural circuits and to study computations in neuronal populations,. However, the
acquired signal is only an indirect measurement of neural activity due to the comparatively
slow dynamics of fluorescent calcium indicators. Different algorithms for estimating spike
rates from noisy calcium measurements have been proposed in the past–, but it is an open
question how far performance can be improved. Here, we report the results of the …