The study was conducted to determine the efficacy of chelated and conventional mineral supplementation on apparently healthy lactating buffaloes. 60 lactating Marathwadi buffaloes were divided into groups A, B and C and were supplemented with chelated mineral mixture, conventional mineral mixture and non-mineral mixture, respectively. Both mineral mixtures were administered at 30 g orally once daily for 90 days. Results revealed that there was significant improvement in appetite and water intake in the chelated mineral mixture supplemented group than the conventional mineral mixture group. Milk yield was significantly higher in chelated and conventional mineral mixture groups. There were 35, 25 and 10% buffaloes from groups A, B and C which showed first postpartum oestrus, respectively. It was observed that there was significant improvement in the reproductive efficiency of the mineral mixture supplemented buffaloes. There were significant differences in haemoglobin and haematocrit values of groups A and B and highly significant differences between groups A and C and significant difference between groups B and C. There were significant increases in Ca, P and Mg of groups A and B. The copper, iron and zinc levels were significantly higher in chelated mineral mixture supplemented animals than conventional mineral mixture supplemented buffaloes. It is suggested that chelated mineral supplementation is beneficial for lactating animals and is also cost effective.