Methods

31 adult male albino rats were divided into 5 groups. Group I (Control), Group II received GTE, 50 mg/kg daily orally for 4 weeks, Group III received a single intraperitoneal injection of Streptozotocin (STZ), 50 mg/kg, Group IV: received STZ followed by GTE and Group V: received STZ followed by human ADSCs (hADSCs) intravenously.

Results

Multiple acidophilic masses, deformed neurons, Congo red+ ve masses and Caspase 3+ ve neurons were seen in group III, became few in group IV and occasional in group V. Multiple Prussian blue+ ve cells were detected in group V. Some CD44+ ve cells were noticed in group III, became multiple in groups IV and V. The mean area of neurons exhibiting acidophilic cytoplasm, mean area of amyloid plaques and mean area% of Caspase 3+ ve cells indicated a significant increase in group III. The mean area% of CD44+ ve cells recorded a significant increase in group IV.

Conclusions

hADSCs exerted a more marked therapeutic effect on the neurodegenerative changes complicating DM and corresponding to AD.