[HTML][HTML] Congo red staining in digital pathology: the streamlined pipeline for amyloid detection through Congo red fluorescence digital analysis

G Cazzaniga, MM Bolognesi, MD Stefania… - Laboratory …, 2023 - Elsevier
Renal amyloidosis is a rare condition caused by the progressive accumulation of misfolded
proteins within glomeruli, vessels, and interstitium, causing functional decline and requiring
prompt treatment due to its significant morbidity and mortality. Congo red (CR) stain on renal
biopsy samples is the gold standard for diagnosis, but the need for polarized light is limiting
the digitization of this nephropathology field. This study explores the feasibility and reliability
of CR fluorescence on virtual slides (CRFvs) in evaluating the diagnostic accuracy and …
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