Hydroxyapatite (HAp) coatings were prepared on 316L stainless steel (316LSS) substrates by electrochemical deposition in the solutions containing Ca(NO₃)₂·4H₂O and NH₄H₂PO₄ at different electrolyte concentrations. Along with the effect of precursor concentration, the influence of temperature and H₂O₂ content on the morphology, structure and composition of the coating was thoroughly discussed with the help of X-ray diffraction (XRD), Scanning electron microscopy (SEM), Fourier transform infrared (FTIR) spectra. The in vitro tests in simulated body fluids (SBF) were carried out and then the morphological and structural changes were estimated by SEM and electrochemical techniques (open circuit potential, polarization curves, Nyquist and Bode spectra measurements). Being simple and cost-effective, this method is advantageous for producing HAp implant materials with good properties/characteristics, aiming towards in vivo biomedical applications.